Fig. 2. SOC1 expression is upregulated by AGL24 during floral transition. (A) Induced expression of AGL24 (left) and SOC1 (right) in 9-day-old pER22-AGL24 Arabidopsis seedlings treated with β-estradiol or mock-treated for 0, 2, 4, 8, 12 and 24 hours. (B,C) Relative temporal expression of SOC1 (B) and AP1 (C) in developing seedlings with different genetic background under long-day conditions. (D) Relative temporal expression of SOC1 in the aerial part without leaf and leaf of agl24-1 and wild-type seedlings. Transcript levels in A-D were determined by quantitative real-time PCR analyses of three independently collected samples. Results were normalized against the expression of TUB2. Error bars indicate s.d. (E) In situ localization of SOC1 at the shot apex of 11-day-old agl24-1 and wild-type seedlings. For the purpose of comparing signals, sections of these plants were placed on the same slides for hybridization and detection. Scale bars: 25 µm.