Fig. 7. Gibberellin (GA) regulates flowering time through independently controlling AGL24 and SOC1. (A) Temporal expression of SOC1 in wild-type and agl24-1 Arabidopsis seedlings with or without GA treatment under short-day conditions. (B) Temporal expression of AGL24 in wild-type and soc1-2 seedlings with or without GA treatment under short-day conditions. Time points on the x-axis indicate the time of collection of plant materials after first GA treatment. Transcript levels in A and B were determined by quantitative real-time PCR analyses of three independently collected samples. Results were normalized against the expression of TUB2. Error bars indicate s.d. (C) Flowering time of soc1-2 and agl24-1 mutants with or without GA treatment under long-day conditions. (D) Flowering time of soc1-2 and agl24-1 mutants with or without GA treatment under short-day conditions. Number of total leaves represents flowering time in C and D. Values representing the mean±s.d. were scored from at least 20 plants of each genotype. Asterisk indicates that flowering was not observed in soc1-2 agl24-1 under short-day conditions without GA treatment.