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Figure 2


Fig. 2. Shroom3 recruits endogenous ROCK to the apical cell junctions in an ASD2-dependent way. (A) Localization of endogenous Rock1 in MDCK-Tet-Off cells expressing FLAG-tagged Shrm-Full or Shrm-{Delta}ASD2. Endogenous Rock1 is visible along the apical cell junctions only in the presence of Shrm-Full [Dox(-)]. Scale bars: 10 µm. (B) Interaction of endogenous Rock1 or Rock2 with Shroom3 in cells. Lysates of the above cells were subjected to immunoprecipitation with anti-FLAG antibody, and then immunoblotted with antibodies against Rock1, Rock2, Lamin B1 and FLAG.