Fig. 5. Expression of RII-C1 in the neural tube antagonizes the apical localization of Rock1 as well as tube closure. Chicken embryos were electroporated with the expression vectors for EGFP, EGFP-tagged RII-C1 or EGFP-tagged RII-C2, and fixed at stage 9. A section of an embryo at the forebrain-midbrain level was double-immunostained for EGFP and Rock1, and adjacent sections were immunostained for pMLC and ZO-1 in each experiment. The neural tube fails to close at the side where the transfection of RII-C1 was more efficient. In this region, the apical levels of Rock1 and pMLC are downregulated. Scale bars: 20 µm.