Fig. 7. Expression of RII-C1 disturbs cell arrangement at the apical surface of the neural tube. Confocal views of triple-immunostaining for EGFP, pMLC and ZO-1 at the apical surface of the neural tubes electroporated with EGFP, EGFP-tagged RII-C1 or RII-C2 and incubated as in the experiments for Fig. 5. Cells displaying the rosette configuration, which were defined as in Fig. 6E, are encircled. No such rosettes are detectable in the RII-C1-positive layer. Arrows indicate residual pMLC signals on which some cells converge, but not assuming the complete rosette configuration as defined above. The bar graph shows results of quantification of pMLC-positive cells. All EGFP-positive cells in randomly selected fields (30-102, 72-132 and 95-178 cells/field for RII-C1, RII-C2 and EGFP, respectively) were counted. Histogram is the average of three different fields from three chicken embryos, and bars represent the standard deviation. ^**P<0.01 against EGFP-transfected embryos. Scale bars: 10 µm.