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Figure 5


Fig. 5. Suppression of 35S::IDA phenotype in bop1 bop2. (A-D) Arabidopsis flowers from positions 3, 7 and 10. (A) Wild type (C24) shows floral organ abscission by position 7. (B) 35S::IDA (C24) with premature abscission of floral organs by position 3. (C) bop1 bop2 lacks floral organ abscission. (D) 35S::IDA bop1 bop2 (Col-0) showing no floral organ abscission. (E) Cauline leaves do not abscise in bop1 bop2. (F) Cauline leaves of 35S::IDA show ectopic abscission. (G) 35S::IDA bop1 bop2 cauline leaves resemble bop1 bop2. (H) RT-PCR from cDNA derived from rosette leaves, buds and flowers from bop1 bop2 and 35S::IDA bop1 bop2. Upper panel shows RT-PCR with IDA primers. IDA is expressed in bop1 bop2 flowers only. 35S::IDA bop1 bop2 plants show expression in rosette leaves, buds and flowers. lower panel, RT-PCR of ACTIN2-7 (ACT) as a positive control. Lane 1 is a genomic PCR control (g). l, leaves; b, buds; f, flowers.