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Figure 2


Fig. 2. dFMRP protein and mRNA are differentially developmentally regulated. (A) Quantitative RT-PCR of dfmr1 mRNA levels normalized to GAPDH2 and reported as fold changes relative to the first time point. Bars show mean±s.e.m. (B) Western blot analysis of dFMRP protein. Each lane represents a single head at indicated stages (2 heads per stage). {alpha}-Tubulin is the loading control. (C) Immunocytochemistry of dFMRP in control (w1118) brains. CB, central brain; OL, optic lobe. Scale bar: 100 µm. Stages: P3, 60-70 hours APF; P4, 88-96 hours APF; 0-3h, 0-3 hours AE; 1d, 21-24 hours AE; 4d, 96-112 hours AE; 7d, 168-184 hours AE; 9d, 216-232 hours AE.