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Figure 4


Fig. 4. Single-cell MARCM clonal analysis of MB gamma neuron development. (A) Representative image of Fasciclin II (FASII, red) labeled Mushroom Body containing a single MARCM gamma neuron clone (green). The white box highlights the area of axonal projection. (B) Developmental profile of axon projections of single-cell MARCM gamma neuron clones. Boxed insets are magnifications of areas of small (<5 µm) presynaptic branches (arrowheads), which are subject to pruning. P3, 60-70 hours APF; P4, 88-96 hours APF; 0-3h, 0-3 hours AE; 4d, 96-112 hours AE. Scale bar: 10 µm.