Fig. 8. Sensory-deprivation modifies dFMRP-dependent axon pruning. (A) Representative images of single-cell MARCM gamma neuron axon projections at 4 days post-eclosion, from animals raised in standard conditions (top row), sensory-deprived conditions (middle row) and sensory-deprived conditions followed by 3 hours of normal sensory stimulation (bottom row). Boxed insets highlight small (<5 µm) branches (arrows). Scale bar: 10 µm. (B) Diagram of dFMRP-dependent changes in MB axonal projections. dFMRP protein and mRNA are expressed maximally during late pupation and the early-use period after eclosion. Activity-dependent pruning in MB axons occurs during this window, dependent on dFMRP. At maturity, transcriptional and translational regulation of dfmr1 becomes uncoupled, as mRNA levels inversely correlate with protein levels.