Fig. 2. Efficient DTA-mediated ablation of Myf5-derived cells at E15.5. Myf5-Cre mice were crossed either to Z/AP reporter mice (A,C,E) or to Z/AP//DTA mice (B,D,F). AP staining (red) reveals Myf5-derived cells in different muscles of Myf5-Cre//Z/AP mice at E15.5 (A,C,E). Simultaneous activation of DTA by Myf5-Cre leads to a complete ablation of Myf5-derived cells in Myf5-Cre//DTA//Z/AP mice (B,D,F). (A,B) tongue muscles. (C-F) Limb and body wall muscles. Scale bars: 200 µm.