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Figure 3


Fig. 3. Loss of MyHC-expressing skeletal muscle cells in myogenin-Cre//DTA mice at E18.5. (A-L) Immunofluorescence staining with a MyHC antibody (MF20) of sections from Myf5-Cre//DTA (A,D,G,J), myogenin-Cre//DTA (B,E,H,K) and wild-type mice (C,F,I,L) at E14.5 (A-C) and E18.5 (D-L). Note the absence of MyHC-positive muscle cells in myogenin-Cre//DTA but not in Myf5-Cre//DTA mice, which show a virtually normal formation of skeletal muscles at E18.5. Sections from wild-type mice are shown for comparison. Scale bars: 200 µm.