Fig. 5. Deletion of Foxd3 in the NC has subtle infrequent effects on heart development. (A) A section through a lineage-labeled control mouse embryo at 9.5 dpc shows NC entering the developing heart (arrows). Box indicates area enlarged in inset. (B) In a control embryo at 10.5 dpc, NC cells are located in PAs 3 and 4 (arrow) and have migrated into the heart (between arrowheads). (C) At 9.5 dpc, a section through a Foxd3 mutant embryo shows NC entering the developing heart (arrows). Asterisk marks blood inside the heart. (D) In a mutant embryo at 10.5 dpc, very few NC cells are migrating into the heart (arrowhead) and very little NC is detected in PAs 3 and 4 (arrow). (E-H') Corrosion casts (E-H) and corresponding traces (E'-H') of 16.5 dpc cardiac outflow tract and associated vessels. The majority of Foxd3 mutants (13 of 17) are indistinguishable from controls (E,F). A few mutants had a duplicated left carotid artery (3 of 17) (G) and one mutant had a PTA (H). Ao, aorta; bca, brachiocephalic artery; dAo, dorsal aorta; DCA, duplicated carotid artery; lca, left carotid; lsa, left subclavian artery; PT, pulmonary trunk; PTA, persistent truncus arteriosus; rca, right carotid artery; rsc, right subclavian artery; ^*, ductus arteriosus.