Fig. 6. Cell migration, survival and proliferation effects in Foxd3 mutants. (A,B) Sections through lineage-labeled 9.5 dpc control and mutant mouse embryos show that NC cells have migrated ventrally to form the DRG. Samples stained as in Fig. 5. (C,D) A more ventral view of the same region pictured in A and B shows NC populating the foregut in the control embryo (arrow) but halting migration near the dorsal aorta in the Foxd3 mutant (arrowhead). (E-H) Control and mutant DRG contain differentiated neurons and glia at 14.5 dpc as indicated by β III tubulin (Tuj1) and Fabp7 (B-FABP) expression. (I,J) Marked changes in cell death occur in the dorsal spinal cord of 9.5 dpc Foxd3 mutant embryos (arrows). (K-N) Whole 9.0 dpc embryos incubated in LysoTracker Red to indicate dying cells. Note pronounced cell death in the mutant hindbrain and migrating NC (arrowheads). At 10.5 dpc (M,N), the mutant has fewer apoptotic cells in the distal region of the PAs (arrowheads), but increased apoptotic cells in the tail (arrows). The overall loss of PA tissue is apparent. (O-R) Matched sections from control and mutant rostral (O,Q) and caudal (P,R) outflow tracts with lineage label (blue) and pH3 immunostaining (brown) showing reduced NC in the Foxd3 mutant outflow tract. Box indicates area enlarged in inset. Arrowheads in inset indicate pH3-positive NC cells.