Fig. 2. The disrupted gene in the dimC^- mutant is a GATA transcription factor. (A) Schematic of the site of insertion of the pBSRBam disruption vector in the dimC^- mutant. The numbers refer to Dictyostelium database identifiers (www.dictybase.org). DDB0217990 is predicted to encode a ubiquitin ligase and DDB0220470 a GATA family transcription factor. (B,C) Quantitative PCR analysis of DDB0217990 (B) and DDB0220470 (C) gene expression in developing wild-type (grey bars) and dimC^- mutant cells (black bars). PCR reactions were performed in triplicate and data averaged. Identical results were obtained with mRNA extracted from two independent developmental time courses.