Fig. 3. GtaC activity is regulated by DIF. (A) gtaC expression is DIF inducible and dependent on DimA and DimB. Expression of gtaC was measured by qPCR in wild-type, dimA^- and dimB^- cells with and without a 1 hour DIF treatment. Results shown are an average of three independent experiments. (B) dimA and dimB expression is also DIF inducible in wild-type cells. Transcript levels were measured by qPCR with and without a 1 hour DIF treatment. Results shown are an average of three independent experiments. (C) GtaC shows DIF-dependent nuclear localization. GtaC-GFP-expressing cells were starved for 4 hours before induction with DIF. Maximal nuclear localization can be seen after 5 minutes. After 15 minutes, nuclear accumulation is no longer visible. (D) Quantification of the kinetics of GtaC-GFP nuclear import in response to DIF. Results shown are an average of three independent experiments.