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Figure 1


Fig. 1. Dynamic localisation of GFP-PCNA in Dictyostelium cells. (A) Localisation of GFP-PCNA during and after mitosis. Timing is in minutes relative to mitosis. Arrows indicate appearance of a nuclear peripheral GFP-PCNA spot. (B) Nuclear GFP-PCNA peaks after mitosis and decays in G2. Bars reflect s.d. (C) Comparison of BrdU incorporation (red) with GFP-PCNA localisation (green); DAPI (blue). Two examples are shown for small foci and one-spot nuclei. Arrow indicates a peripheral BrdU spot colocalised to the GFP-PCNA spot, as observed in a number of cells (see table).