Fig. 7. Mitosis precedes S phase after spore germination. (A) Percentage of BrdU-positive cells after spore induction with DMSO; labelled with 100 µM BrdU for 30 minutes. Bars reflect s.d.; three replicates. (B) Onset of mitosis after spore germination. Percentage of cells dividing in 2-hour periods. First divisions occur in the 18-20 hour window. Two replicates. (C) Imaging-relative timing of division and S phase after spore germination, using GFP-PCNA as an S-phase marker. Stills from a movie of a germinated spore undergoing mitosis (arrows) are shown. Time after induction of germination (hours) is indicated.