Fig. 4. The Antp^Ctx eye-antennal disc is transformed into various parts of the wing disc. (A) Su(H)-lacZ staining on an Antp^Ctx eye-antennal disc shows ectopic N signaling (arrow). (B) Su(H)-lacZ staining on wild-type eye-antennal discs showing active Notch signaling. (C) Antp^Ctx eye-antennal disc showing ectopic VG protein (red) and ectopic ANTP protein (green). (D,F,H,J) Antp^Ctx eye-antennal disc. (E,G,I,K) Wild-type eye-antennal disc. (D,E) Ectopic VG (green) represses EYA (red) cell non-autonomously in the Antp^Ctx eye-antennal disc (D). (F,G) Ectopic VG (green) and ectopic EYG (eyg-lacZ; β-Gal in red) in the Antp^Ctx eye-antennal disc (F) indicate a transformation toward notum identity. (H,I) Ectopic VG (green) is co-expressed with ectopic WG (wg-lacZ; β-Gal in red) in the Antp^Ctx eye-antennal disc (H). The localization of the ectopic VG protein corresponds to the dorsal eye region where the ectopic wings are formed. (J,K) Ectopic WG (red) is co-expressed with ectopic DPP (dpp-lacZ; β-Gal in red) in the Antp^Ctx eye-antennal disc, indicating transformation to wing disc cells (J).