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Figure 4


Fig. 4. TuJ1 (green) and QCPN (red) immunostaining of E7 3-6 ablated + Q3 whole-mount gut preparations. (A-D) TuJ1 immunolabelling of (A) stomach, (B) duodenum, (C) umbilicus and (D) the migration wave-front at the end of the cecal buds (arrow). (E-I) Confocal images showing the extensive contribution of quail NCC in the gut regions. The majority of TuJ1+ cells are stained with QCPN in the (E) stomach, (F) duodenum, (G) umbilicus and (H) cecal buds. Arrowheads indicate TuJ1+QCPN-cells. (I) TuJ1 immunostaining in the NoR in the hindgut. NoR, Nerve of Remak; umb, umbilicus; cb, cecal buds; hg, hindgut. Scale bar in E: 50 µm.