Fig. 5. TuJ1 (green) and QCPN (red) immunostaining of E10 3-6 ablated + Q3, 3-6 ablated + Q sacral and 3-6 ablated + Q trunk whole-mount gut preparations. (A) Characteristic ENS network and perivascular projections in the stomach. (B,C) The duodenum (B) and the umbilicus level (C) contain extensive interconnected ganglia. (D) The cecal buds and hindgut are completely colonised by NC-derived cells. Similar immunostaining was also observed in 3-6 ablated + Q sacral and 3-6 ablated + Q trunk guts. (E-I) 3-6 ablated + Q3 guts showing extensive colonisation by quail NCC within (E) the duodenum, (F) small intestine, (G) umbilicus, (H) cecal buds and (I) hindgut. (J-N) 3-6 ablated + Q sacral gut. The coincidence of TuJ1 and QCPN within (J) the duodenum and (K) small intestine. Fewer quail NCC were observed within the TuJ1+ ENS network. Only occasional QCPN+ cells were present within the umbilicus region (L). No quail cells were present in the cecal bud region (M) or hindgut (N). (O-S) Immunolabelling of 3-6 ablated + Q trunk guts reveals that there are no quail NCC throughout the entire gut. Scale bar in E: 100 µm.