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Figure 8


Fig. 8. QCPN (red) and BrdU (green) immunolabelling with DAPI staining (blue) of E4.5 1-7 ablated + Q3 embryos. (A,D) QCPN staining of NCC that have migrated from the grafted neural tube (not shown) towards the developing foregut. (B,E) BrdU detects proliferating cells within the embryo and within the NCC adjacent to the foregut, respectively. Inset in E shows QCPN and BrdU labelled cells. (C,F) Merged images of (C) A,B and (F) D,E with DAPI staining. Bracketed region in C shows the remnants of the neural tube following ablation. Arrowheads (E,F) indicate proliferating quail NCC. fg, foregut. Scale bars: 200 µm in A; 50 µm in D.