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Figure 1


Fig. 1. Characterization of lymphatic endothelial cells in wild-type developing axillary lymph nodes. Lymph nodes (LNs) were examined at E14.5 (A,C,E,G) and E16.5 (B,D,F,H). (A,B) LNs were identified by combined staining for CD4 (green), which is expressed by LTi cells, IL7R{alpha} (red), which is expressed by LTi cells and their precursors, and CD45 (blue), which is expressed by all hematopoietic cells. (C-H) Subsequent sections were stained to detect (C,D) the lymphatic endothelial cell (LEC) marker LYVE1(blue) in combination with the stromal marker MAdCAM1 (red) and the endothelial cell marker VE-cadherin (green), (E,F) the LEC marker PROX1 (green) in combination with the LN stromal cell marker podoplanin (red) and the vascular endothelial cell marker VEGFR3 (blue), and (G,H) the vascular endothelial cell markers VEGFR2 (green), MECA32 (red) and VEGFR1 (blue). Arrows in C and E indicate the lining of the lymphatic endothelium. Arrowheads in G and H indicate blood vessels. Scale bars: 75 µm in A-H.