Development -- Vondenhoff et al. 136 (1): 29 Figure IG4

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Fig. 4. Lymph node anlagen are present in E17.5 Prox1 conditional-null mouse embryos. (A,B) Staining of wild-type and conditional-null (Tie2-Cre/Prox1^flox/flox) brachial LNs with antibodies against LYVE1 (green), MAdCAM1 (red) and CD4 (blue) revealed that LYVE1⁺ LECs that coexpress MAdCAM1 are absent from the LN anlagen of Tie2-Cre/Prox1^flox/flox embryos. (C,D) Staining of adjacent sections with antibodies against MAdCAM1 (green), VCAM1 (red) and podoplanin (blue) indicated that the MAdCAM1⁺ VCAM1⁺ stromal organizer cells, which are abundant in the wild-type LN anlagen (C), are greatly reduced in the Tie2-Cre/Prox1^flox/flox LN anlagen (D). Scale bars: 75 µm in A-D.