Fig. 2. Glial division around the antennal nerve after eclosion. (A,B) An adult fly brain of the wild-type (CS) strain after 10 days of BrdU treatment. Projection of confocal optical sections of the frontal half of the brain (A) and high-magnification single section of the right hemisphere around the antennal nerve (B). BrdU-positive cells were found predominantly around the antennal nerve (arrowheads). (C) Percentage of the antennal nerves associated with BrdU-positive cells. The colour coding indicates the percentage of antennal nerves with the indicated numbers of labelled cells. The sample number (n) is indicated above each bar. (D) Pulse-chase labelling experiment. Blue and orange bars show the percentage of antennal nerves with the indicated numbers of BrdU-positive cells. (E) Percentage of antennal nerves with lacZ-positive cells induced by MARCM. -, flies without heat shock; +, flies with heat shock to induce MARCM clones. ^**, P<0.01 (Fisher's exact test). (F) A MARCM-labelled cell (expressing β-galactosidase, arrowhead) also incorporated BrdU. Flies were heat shocked 1 day after eclosion and fixed 4 days later. Frontal optical section around an antennal nerve. A merged image (left) and single-channel images (insets) are shown. Scale bars: 50 µm in A; 10 µm in B,F.