Fig. 3. Glial cell division induced by neural PCD. (A) The percentage of cells that are REPO-positive or -negative among the BrdU-positive cells observed around the antennal nerve after a 3-hour BrdU treatment (0-3h BrdU). The sample number (n) is indicated above the bar. (B,C) Frontal optical section around an antennal nerve. Dorsal is up. A merged image (left) and single channel images (insets) are shown. (B) A BrdU-positive cell expressed the glial marker REPO, but not the neural marker ELAV (arrowhead). (C) Morphology of a BrdU-positive REPO-positive glial cell visualised by MARCM (expressing GFP, arrowhead). Flies were heat shocked 1 day after eclosion and fixed 4 days later. (D,E) Specimens after 10 days of BrdU treatment. Projection of confocal optical sections around the antennal nerves (left), and a single optical section of the ovary (right). (D) Wild-type (CS) flies. BrdU-positive cells were observed both in the brain (arrowheads) and ovary. (E) The elav-p35 strain, in which neuronal PCD was inhibited by continuous ectopic expression of p35 in neurons. BrdU incorporation in the brain was suppressed specifically. Scale bars: 10 µm.