Fig. 5. Wnt signaling promotes the generation of neural crest cells by inducing
Bmp4 expression. Relative Bmp4 and Sp5 mRNA
levels measured by quantitative real-time PCR in stage 2 M chick explants
cultured in the absence (M) or presence (M+Wnt3A) of Wnt3A, or in stage 2 B
explants cultured in the absence (B) or presence (B+Frz) of mFrz8CRD. Bars
represent mean ± s.e.m. of five independent experiments.
*P<0.05, **P<0.01; two-sided,
one-sample t-test.