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Figure 1


Fig. 1. A spontaneous mutant mouse strain, abnormal lymphatics (al), and positional candidate cloning. Chylous ascites (A) and blood-filled vessels (B) in mutant newborns. Blood-filled vessels were Lyve1+ lymphatic vessels (C, arrows). Arrowheads indiacte blood vessels. Scale bar: 100 µm. (D) Genotyping of the 139 affected mice (n=2,4,5,128) using SSLP markers mapped the al locus to a B6-derived region between D8Ims10 and D8Ims18. (E) Sequencing of PCR-amplified genomic DNA showed that a G-to-A transitional mutation (an asterisk in D) in the Plcg2 gene occurred in the affected mice. (F) Western blot analysis of newborn heart tissue lysates showed that PLC{gamma}2 was not expressed in al/al mice. PLC{gamma}1, loading control.