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Figure 2


Fig. 2. Aberrant lymph sacs in PLC{gamma}2-null embryos. (A-F) Transverse sections of E13.5 embryos immunostained for Lyve1 (green) and CD31 (red). D-F are higher magnifications of A-C, respectively. (C,F) Aberrant separation of a lymph sac from a cardinal vein. Blue, DAPI-stained nuclei; A, carotid artery; V, cardinal vein; L, jugular lymph sac. Arrow indicates a site at which ECs of the two vessels are in contact. Arrowheads indicate connections between BECs and LECs. Scale bars: 200 µm in A-C; 50 µm in D-F.