Fig. 2. Accumulation of DeltaD protein on the plasma membrane in the mib mutant. (A,B) Hindbrain expression of deltaD transcript in wild-type (WT) and mib^ta52b 10-somite stage (10 ss) zebrafish embryos. (C,D) DeltaD protein in areas corresponding to the rectangles in A and B. Embryos are stained with zdD2. The patches of DeltaD expression in D are in rhombomeres 3, 4 and 5. (E,F) deltaD transcript in wild type and mib^ta52b at the tail bud stage (tb) in hindbrain neurogenic domains. Area shown here corresponds approximately to rectangular areas in Fig. S1A,B (see supplementary material). mib^ta52b embryos do not have significantly exaggerated deltaD expression. (G,H) zdD2 staining in corresponding wild-type and mib^ta52b neurogenic domains. In mib^ta52b mutants, most DeltaD is at the cell surface, even when expression is not so exaggerated.