Fig. 7. Mib1, Notch1a and Notch3 are not essential for DeltaC endocytosis. (A-C') Distribution of DeltaC in wild-type zebrafish embryos (A,A') and in two mib alleles, mib^m178 (B,B') and mib^ta5ab (C,C'). (D,D') Distribution of DeltaC in Notch MO embryos. Eight-somite stage embryos were double stained with zebrafish DeltaC antibody zdC1 (grey in A-D, green in A'-D') and β-catenin (red in A'-D'). A dorsal view of a lateral neurogenic domain in the caudal neural plate is shown. In WT embryos, only subset of cells express DeltaC in cytoplasmic puncta (A,A'). A neurogenic phenotype allows more cells to express DeltaC in both mib mutants and Notch MO embryos as Notch signaling is reduced (B-D). However, DeltaC distribution remains cytoplasmic in mib^m178 (B,B') and Notch MO embryos (D,D'), and it only accumulates on the cell surface in mib^ta52b mutant embryos (C,C').