Fig. 7. Hipk phosphorylates Arm. (A) HEK293T cell lysates expressing the indicated constructs were immunoprecipitated with appropriate antibodies and the purified proteins were subjected to in vitro kinase assays. Arm is phosphorylated in the presence of Hipk WT (lane 1), but not with Hipk KD (lane 2). Relative levels of protein used in the kinase assay were visualized by immunoblotting (IB) with indicated antibodies. (Lanes 3-11) Indicated truncations of Arm were subjected to kinase assays and loading controls are indicated. (B) Schematic of the Arm truncations used in the study.