Fig. 8. Drosophila Hipk/mouse Hipk2 enhance Wg/Wnt responsive transcription in vitro. (A) Topflash assays in HEK293T cells showed promotion of Drosophila Tcf/Arm-dependent transcription by Hipk in a kinase-dependent manner. Topflash values are indicated on the left in black columns. These values were from the average of three independent transfection experiments. Vectors used for each experiment are as indicated in the figure. The negative control Fopflash values are given on the right in white columns. (B) Hipk2 promotes Lef1-mediated transcription in a kinase-dependent manner. Hipk can also stimulate Topflash in HeLa cells. Transcriptional assays were performed with vertebrate homologues in HeLa cells. Indicated values represent the average of two independent transfection experiments. Results are labeled according to those described in A. (C) Topflash assays were performed in Drosophila S2R+ cells in the absence (lanes 1-3) or presence of Wg-conditioned media (lanes 4-6). Both Hipk (lanes 2, 5) and Hipk2 (lanes 3, 6) enhanced Topflash under both conditions.