Fig. 9. Hipk enhances the stability of Arm. (A) Cell lysates from discs expressing omb>hipk showed elevated Arm protein when compared with wild type. (B) Lysates from S2R+ cells transfected with Hipk2 show elevated Arm, compared with control. (C) Protein lysates from HeLa cells transfected with Hipk2 and β-catenin show elevated levels of β-catenin compared with control and after transfection with mouse Hipk2 KD. (D) HEK293T cells expressing the indicated constructs were treated with the protein translational inhibitor cycloheximide (CHX). Whole cell lysates were collected over several time points after treatment and analyzed by western blot. Arm levels were visualized by immunoblotting with anti-HA antibody. Co-expression of Hipk WT, and to a lesser degree Hipk KD, enhanced the stability of Arm. β-Tubulin was used as a loading control.