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Figure 2


Fig. 2. debcl regulates developmental cell death in the nervous system. (A,B) TUNEL staining is moderately reduced in debcl mutant embryos (B) compared with WT (yw) embryos (A; stage 11). (C-H) The presence of extra cells during embryonic development was examined by {alpha}-Kr Ab staining (C-G) and slit-lacZ staining (I,J slit-lacZ reporter; K,L, {alpha}-βGal Ab staining). Arrows in J-L indicate extra slit-lacZ+ cells. Dashed lines in E and H represent the WT cell count threshold for each segment surveyed. (E) Quantification of extra cells in the VNC by {alpha}-Kr Ab. Error bars show ±s.d. (n=6). (H) Quantification of cells in Bolwig's Organ. Error bars show ±s.d. (n=8). (M) Quantification of VNC cells by {alpha}-βGal Ab staining. Error bars show ±s.d. (n=8).