Fig. 8. Development of dA1 neurons in Olig3 mutant mice. Analyses of rhombomere 7 of control (A,D,G) and Olig3 mutant (B,E,H) mice. (A,B) Immunohistological analysis using anti-Lhx2/9 antibodies at E11.5. (C) Quantification of Lhx2/9⁺ neuron numbers. (D,E,G,H) In situ hybridization analysis using a probe specific for Barhl1. Arrows indicate external cuneate nuclei in D,E, and the lateral reticular nuclei in G,H. (F,I) Morphometric analysis of the volume of external cuneate (F) and lateral reticular nuclei (I) of control (+/-) and Olig3 mutant mice (-/-). Scale bars: 50 µm in B; 100 µm in H.