Fig. 3. PP4 RNAi elevates the Hh-induced Smo cell-surface accumulation. (A-P) CFP-tagged Smo constructs were transfected into S2 cells and treated with phosphatase inhibitor (OA), phosphatase dsRNA or Hh-conditioned medium. Cell-surface-localized Smo was visualized by immunostaining with anti-SmoN antibody. The total amount of expressed Smo was indicated by the CFP signal. (Q) Ratio of cell surface level to total level of Smo. Each data set was based on 15 individual cells. WT, CFP-Smo; SD123, CFP-SmoSD123; 626-678, CFP-Smo 626-678.