Fig. 2. Distribution of the RIM-BP3 protein in elongating spermatids. Testicular germ cells were prepared from adult wild-type mice, and indirect immunofluorescence staining was performed using polyclonal anti-RIM-BP3 SbcC (green) and monoclonal anti--tubulin (red) antibodies. The slides were counterstained with DAPI to visualize the nucleus. Testicular germ cells from RIM-BP3 knockout mice were used as negative controls (see Fig. S2 in the supplementary material). Scale bar: 10 µm.