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Figure 2


Fig. 2. A C-terminal truncated form of Celsr2 (Celsr-{Delta}C) is dominant negative and Celrs regulates epiboly independently of the PCP. Wild-type (A,B,D,E) or MZord embryos (C,F) were injected with 300 pg (H, high) (A,D) or 150 pg (L, low) (B,C,E,F) RNA encoding Celsr-{Delta}C-HA were visualised at 90% epiboly with phalloidin for actin (A-C) or anti-{alpha}-catenin (D-F). Arrowheads indicate the leading edge of deep cells, showing epiboly defects with deep cells retracted from the leading edge of the EVL. (G-I) MZord embryos (G) or MZord embryos injected with 0.4 pmoles stbmMO (H) or 0.75 pmoles dvl2MO (I) were visualised at 90% epiboly with anti-{alpha}-catenin. Dorso-posterior views of 90% epiboly embryos. Anterior is upwards and the antibodies used are indicated in the bottom right-hand corner. MZord embryos injected with stbmMO or dvl2MO show normal epiboly movements, as assessed by closure of the germ ring blastopore.