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Figure 7


Fig. 7. Differential cell cohesive properties of wild-type cells compared with cells from celsr mutant/morphants and cells from embryos expressing Celsr-{Delta}C. Wild-type and MZord embryos were injected with RNA/morpholinos together with fluorescein-dextran (FDX, green) or rhodamine-dextran (RDX, red), as indicated. (A) 30-40% epiboly embryos were dissociated and dissociated cells from different populations were mixed in a minimal volume of a hanging drop and kept overnight to analyse aggregates by confocal microscopy. (B) Cells from wild-type embryos (green) and wild-type embryos (red). (C) Cells from MZord embryos injected with 0.4 pmoles each of celsr1a and celsr1b morpholinos (green) and wild-type embryos (red). (D) Cells from wild-type embryos injected with 300 pg Celsr-{Delta}C-HA RNA (green) and wild-type embryos (red). (E) Cells from wild-type embryos injected with 100 pg Lyn-Celsr RNA (green) and wild-type embryos (red). Celsr mutant/morphant cells (C) or Celsr-{Delta}C-expressing cells (D) are strongly segregated from wild-type cells, whereas Lyn-Celsr-expressing cells are only weakly segregated from wild-type cells (E).