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Figure 2


Fig. 2. Expression of netrin 1 and Dcc by oligodendrocytes in vitro. (A,A') Netrin 1 protein (green) on the surface of myelin-like membrane sheets of non-permeabilized mature rat oligodendrocytes in culture. Recombinant netrin 1, labeled using a Myc epitope tag, preferentially localizes to branches formed by the cells (arrow). (B,B') Dcc (green) distributed along oligodendrocyte processes and at puncta along the edge of Mbp-immunopositive (red) myelin-like membrane sheets (arrow). A,B, 40x0.5 n.a. objective. Scale bars: 40 µm in A'; 20 µm in B'.