Fig. 9. Treatment of mature oligodendrocytes with netrin 1 inhibits RhoA activity (A) Levels of GTP-bound RhoA in rat oligodendrocytes were assessed using GST-rhotekin pulldown. Treatment of mature oligodendrocytes with netrin 1 (100 ng/ml, 5 minutes) caused a significant decrease in RhoA-GTP compared with controls. (B,C) Similar treatment of cells resulted in no significant change in the levels of GTP-bound Cdc42 or Rac1, as assessed by GST-Pak-CRIB pulldown. (D) Addition of netrin 1 to mature oligodendrocytes (100 ng/ml, 5 minutes) did not alter the amount of N-WASP recruited to Dcc. ^*P<0.05 versus control. Error bars indicate s.e.m.