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Figure 2


Fig. 2. Ectopic cartilage formed in Col2.3-HSD2-transgenic mice. (A,B) Skeletal preparations with cartilage stained by Alcian Blue. (A) Wild-type P1, lateral and dorsal views. (B) Col2.3-11βHSD2 transgenic littermate of A. The cartilage remnants are indicated by arrows. P, parietal bone; IP, interparietal bone. (C,D) Parietal bones, apical segment. (C) Wild type; (D) Col2.3-11βHSD2 transgenic littermates. Osteoblasts are denoted by arrows. Toluidine Blue staining. Scale bars: 25 µm. (E,F) Parietal bones, base segment. (E) Wild type; (F) Col2.3-11βHSD2 transgenic littermates. Toluidine Blue staining. Scale bars: 100 µm. (G,H) Parietal bones, base segment. (G) Wild type; (H) Col2.3-11βHSD2 transgenic littermates. Alkaline phosphatase (ALP) staining. Scale bars: 100 µm. (I) The boxed region in G is enlarged to show ALP+ve pre-osteoblastic cells (arrows). Ca, cartilage. Scale bar: 25 µm. (J) The boxed region in H is enlarged to show ALP+ve osteoblastic cells (arrows) but lack of spindle shaped ALP+ pre-osteoblastic cells. Ca, cartilage. Scale bar: 25 µm.