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Figure 3


Fig. 3. Cranial skull defects in P7 Col2.3-11βHSD2 transgenic mice. (A,B) Skeletal preparations, cartilage stain (Alcian blue). (A) Wild type; (B) Col2.3-11βHSD2 transgenic littermate of A. Arrows denote ectopic cartilage under the sutures. P, Parietal bone; IP, interparietal bone. (C,D) Parietal bone, top segments. (C) Wild type; (D) Col2.3-11βHSD2 transgenic littermate of (C). Red arrowheads in C and D indicate calvarial bone fronts of the sagittal sutures. The black arrow in D denotes ectopic cartilage under the sagittal suture. Toluidine Blue staining. Scale bars: 100 µm. (E,F) Parietal bone, base segment. (E) Wild type; (F) Col2.3-11βHSD2 transgenic littermate of E. Toluidine Blue staining. Scale bars: 100 µm. (G,H) Parietal bone, base segment at higher magnification. (G) Wild type; (H) Col2.3-11βHSD2 transgenic littermate. Toluidine Blue staining. Scale bars: 25 µm.