Fig. 5. Wnt/β-catenin signaling is reduced in neonatal (P0) transgenic parietal bone and cartilage. (A,B) Immunohistochemistry for β-catenin at regions of overlapping bone and cartilage in P0 parietal bones. Arrows indicate osteoblasts. b, bone; ca, cartilage; br, brain. Scale bars: 50 µm. (C,D) Immunohistochemistry for β-catenin (β-cat) at the sagittal sutures of P0 parietal bones. Red arrowheads indicate bone growth fronts in the sagittal sutures. The black arrows indicate mesenchymal progenitors in the non-ossified suture area. b, bone. Scale bars: 50 µm. (E,F) Parietal bones. In situ hybridization for Wnt9a at an area of overlapping bone and cartilage. b, bone; ca, cartilage. Scale bars: 50 µm. (G) mRNA expression for Wnt9a and Wnt10b in P0 parietal bones. Col2.3-11βHSD2-transgenic mice were bred with Col2.3-GFP mice to generate Col2.3-11βHSD2-GFP-transgenic and Col2.3-GFP littermates. Parietal bones were dissected from P0 Col2.3-11βHSD2-GFP-transgenic and Col2.3-GFP littermates and RNA was isolated. Real-time PCR quantitation of relative mRNA expression levels for Wnt 10b and Wnt9a after normalization by GFP expression. Data are represented as mean±s.e.m. (^*P<0.05 versus wild type, n=6).