Fig. 4. Shortened cycle 11 interphase of mnk nopo-derived embryos. (A,B) Cell-cycle timing during cortical embryonic divisions. Bar graph (A) shows mean cycle 11 interphase lengths for various genotypes. Table (B) summarizes mean cycle 11-13 interphase (I) and mitosis (M) lengths. n, number of embryos. Error bars (A) and ±values (B) represent s.e.m. Single and double asterisks mark interphases significantly shorter than wild type (P<0.01 and <0.001, respectively). (C,D) Immunoblotting reveals normal pY15-CDK1 (C) and Cyclin B (D) levels in mnk nopo^Z1447-derived embryos (1-2 hours). Control grp-derived embryos have reduced pY15-CDK1 levels. Anti--tubulin or anti-GAPDH was used as a loading control.