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Journal Articles |
An intriguing aspect of developmental biology is the extent to which early development is controlled by the spatial arrangement of molecules in the oocyte. Ultraviolet (u.v.) irradiation of the vegetal pole of the fertilized egg of Xenopus laevis affects both the development of the embryonic dorsal axis and also the formation of primordial germ cells (PGCs). However, the importance of cytoplasmic localization in the oocyte has been difficult to assess because, until recently, it has proved impossible to mature and fertilize cultured oocytes routinely. In this report, we describe a method for routinely maturing and fertilizing cultured oocytes of Xenopus. We find that the u.v.-sensitive period for PGC and dorsal axis formation extends back into stage-VI oocytes, thus demonstrating a true oocyte contribution to these processes. This method also allows greater time for experimental intervention and should facilitate the eventual isolation of the molecules concerned.
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J Heasman Patterning the Xenopus blastula Development, January 11, 1997; 124(21): 4179 - 4191. [Abstract] [PDF]
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H Kageura Activation of dorsal development by contact between the cortical dorsal determinant and the equatorial core cytoplasm in eggs of Xenopus laevis Development, January 4, 1997; 124(8): 1543 - 1551. [Abstract] [PDF]
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C Wylie, M Kofron, C Payne, R Anderson, M Hosobuchi, E Joseph, and J Heasman Maternal beta-catenin establishes a 'dorsal signal' in early Xenopus embryos Development, January 10, 1996; 122(10): 2987 - 2996. [Abstract] [PDF]
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C Forristall, M Pondel, L Chen, and M. King Patterns of localization and cytoskeletal association of two vegetally localized RNAs, Vg1 and Xcat-2 Development, January 1, 1995; 121(1): 201 - 208. [Abstract] [PDF]
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J Heasman, D Ginsberg, B Geiger, K Goldstone, T Pratt, C Yoshida-Noro, and C Wylie A functional test for maternally inherited cadherin in Xenopus shows its importance in cell adhesion at the blastula stage Development, January 1, 1994; 120(1): 49 - 57. [Abstract] [PDF]
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T Holowacz and R. Elinson Cortical cytoplasm, which induces dorsal axis formation in Xenopus, is inactivated by UV irradiation of the oocyte Development, January 9, 1993; 119(1): 277 - 285. [Abstract] [PDF]
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 R C Smith, M B Dworkin, and E Dworkin-Rastl Destruction of a translationally controlled mRNA in Xenopus oocytes delays progesterone-induced maturation. Genes & Dev., October 1, 1988; 2(10): 1296 - 1306. [Abstract] [PDF]
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