spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Katula, K. S.
Right arrow Articles by Davidson, E. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Katula, K. S.
Right arrow Articles by Davidson, E. H.

Development, Vol 101, Issue 3 437-447, Copyright © 1987 by Company of Biologists

JOURNAL ARTICLES

Ontogenic expression of a CyI actin fusion gene injected into sea urchin eggs

KS Katula, BR Hough-Evans, RJ Britten and EH Davidson
Division of Biology, California Institute of Technology, Pasadena 91125.

The 5' terminus of the CyI actin gene transcription unit of Strongylocentrotus purpuratus was located by primer extension and other procedures, and the flanking upstream region was partially sequenced and mapped. A fusion gene was constructed containing about 2.5 kb of 5' flanking sequence, the transcribed leader sequence, and the first few codons of the CyI gene ligated to the bacterial gene coding for chloramphenicol acetyl transferase (CAT). This was micro-injected into the cytoplasm of S. purpuratus eggs, and CAT enzyme activity was measured at various stages of embryonic development. CAT synthesis was activated between 10 and 14 h postfertilization, the same time at which newly synthesized transcripts of the endogenous CyI gene first appear. The exogenous CyI.CAT fusion DNA replicated actively during cleavage, as observed previously for other DNAs injected into sea urchin egg cytoplasm. Thus the absence of CAT activity prior to 10 h postfertilization could not be due to insufficient CyI.CAT genes. The amounts of CAT enzyme produced by embryos bearing CyI.CAT deletions that lack various regions of the CyI sequence were measured. As little as 254 nucleotides of upstream CyI sequence suffice for correct temporal activation of the fusion construct, although the level of CAT enzyme produced in embryos bearing any deletion retaining less than 850 nucleotides of upstream sequence was significantly lowered compared to controls bearing the complete CyI.CAT fusion construct.


This article has been cited by other articles:


Home page
 Genes Dev.Home page
P Thiebaud, M Goodstein, F J Calzone, N Theze, R J Britten, and E H Davidson
Intersecting batteries of differentially expressed genes in the early sea urchin embryo.
Genes & Dev., November 1, 1990; 4(11): 1999 - 2010.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
M DiLiberto, Z C Lai, H Fei, and G Childs
Developmental control of promoter-specific factors responsible for the embryonic activation and inactivation of the sea urchin early histone H3 gene.
Genes & Dev., July 1, 1989; 3(7): 973 - 985.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
H M Sucov, B R Hough-Evans, R R Franks, R J Britten, and E H Davidson
A regulatory domain that directs lineage-specific expression of a skeletal matrix protein gene in the sea urchin embryo.
Genes & Dev., October 1, 1988; 2(10): 1238 - 1250.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
R R Franks, B R Hough-Evans, R J Britten, and E H Davidson
Spatially deranged though temporally correct expression of Strongylocentrotus purpuratus actin gene fusion in transgenic embryos of a different sea urchin family.
Genes & Dev., January 1, 1988; 2(1): 1 - 12.
[Abstract] [PDF]


© The Company of Biologists Ltd 1987