Development, Vol 102, Issue 4 749-761, Copyright © 1988 by Company of Biologists

JOURNAL ARTICLES

Neural cell adhesion molecules during embryonic induction and development of the kidney

G Klein, M Langegger, C Goridis and P Ekblom
Friedrich-Miescher Laboratorium der Max-Planck-Gesellschaft, Tubingen, FRG.

The neural cell adhesion molecules (N-CAM) are a family of related glycoproteins with Mr of 180, 140 and 120 x 10(3) (180K etc.). In the embryo, they are often highly sialylated and migrate as a diffuse band of 170-250K. N-CAM are found in non-neural tissues and we have now studied the expression of N-CAM in the developing mouse kidney. During kidney development, a unique conversion of a mesenchyme to an epithelium occurs and it is thought that this is mediated by an increase in cell adhesivity. By immunofluorescence, we show that N-CAM is present already at onset of kidney development on the cells of the uninduced nephrogenic mesenchyme. After induction, when the cells convert into an epithelium, they lose N-CAM gradually and instead begin to express uvomorulin, another primary CAM. By using an organ culture model, we could rather precisely show that N-CAM and uvomorulin are coexpressed for a short period, but, when epithelial cell polarization is evident, only uvomorulin is present on the epithelium, whereas N-CAM is confined to the surrounding mesenchyme. Immunoblotting for N-CAM revealed that the 'embryonic' form of N-CAM, the broad 170-250K band was not present in the embryonic kidney, which instead expressed the three distinct 180K, 140K and 120K bands typical of adult neurones. The 180K and 140K bands were gradually lost during development and were no longer detectable in adult kidneys. By using an N-CAM cDNA, we detected three different mRNAs of 7.4, 6.7 and 4.3 kb in the developing kidney, but this expression was restricted to the embryonic and early postnatal stages. No transcripts were detectable in adult kidneys. The studies do not support the hypothesis that N-CAM expression in the kidney is turned on by embryonic induction. Rather, we suggest that N-CAM are important adhesives for the predetermined, but not yet induced, nephrogenic mesenchyme.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				J. Markovic-Lipkovski, C. A. Muller, G. Klein, T. Flad, T. Klatt, S. Blaschke, J. T. Wessels, and G. A. Muller Neural cell adhesion molecule expression on renal interstitial cells Nephrol. Dial. Transplant., June 1, 2007; 22(6): 1558 - 1566. [Abstract] [Full Text] [PDF]

				Y. S. Kanwar, J. Wada, S. Lin, F. R. Danesh, S. S. Chugh, Q. Yang, T. Banerjee, and J. W. Lomasney Update of extracellular matrix, its receptors, and cell adhesion molecules in mammalian nephrogenesis Am J Physiol Renal Physiol, February 1, 2004; 286(2): F202 - F215. [Abstract] [Full Text] [PDF]

				H.-T. Cheng, J. H. Miner, M. Lin, M. G. Tansey, K. Roth, and R. Kopan {gamma}-Secretase activity is dispensable for mesenchyme-to-epithelium transition but required for podocyte and proximal tubule formation in developing mouse kidney Development, October 15, 2003; 130(20): 5031 - 5042. [Abstract] [Full Text] [PDF]

				J. V. Bonventre Dedifferentiation and Proliferation of Surviving Epithelial Cells in Acute Renal Failure J. Am. Soc. Nephrol., June 1, 2003; 14(90001): S55 - 61. [Abstract] [Full Text] [PDF]

				U. Dahl, A. Sjodin, L. Larue, G. L. Radice, S. Cajander, M. Takeichi, R. Kemler, and H. Semb Genetic Dissection of Cadherin Function during Nephrogenesis Mol. Cell. Biol., March 1, 2002; 22(5): 1474 - 1487. [Abstract] [Full Text] [PDF]

				S. Hosono, I. Gross, M. A. English, K. M. Hajra, E. R. Fearon, and J. D. Licht E-cadherin Is a WT1 Target Gene J. Biol. Chem., April 6, 2000; 275(15): 10943 - 10953. [Abstract] [Full Text] [PDF]

				C. M. Cale, N. J. Klein, P. J. D. Winyard, and A. S. Woolf Inflammatory mediators in human renal dysplasia Nephrol. Dial. Transplant., February 1, 2000; 15(2): 173 - 183. [Abstract] [Full Text] [PDF]

				M. Abbate, D. Brown, and J. V. Bonventre Expression of NCAM recapitulates tubulogenic development in kidneys recovering from acute ischemia Am J Physiol Renal Physiol, September 1, 1999; 277(3): F454 - F463. [Abstract] [Full Text] [PDF]

				R. B. Thomson and P. S. Aronson Immunolocalization of Ksp-cadherin in the adult and developing rabbit kidney Am J Physiol Renal Physiol, July 1, 1999; 277(1): F146 - F156. [Abstract] [Full Text] [PDF]

				E. I. Wallner, Q. Yang, D. R. Peterson, J. Wada, and Y. S. Kanwar Relevance of extracellular matrix, its receptors, and cell adhesion molecules in mammalian nephrogenesis Am J Physiol Renal Physiol, October 1, 1998; 275(4): F467 - F477. [Abstract] [Full Text] [PDF]

				J Davies, M Lyon, J Gallagher, and D Garrod Sulphated proteoglycan is required for collecting duct growth and branching but not nephron formation during kidney development Development, January 5, 1995; 121(5): 1507 - 1517. [Abstract] [PDF]

				U. W. Rothenpieler and G. R. Dressler Pax-2 is required for mesenchyme-to-epithelium conversion during kidney development Development, November 1, 1993; 119(3): 711 - 720. [Abstract] [PDF]

				L Bally-Cuif, C Goridis, and M. Santoni The mouse NCAM gene displays a biphasic expression pattern during neural tube development Development, January 2, 1993; 117(2): 543 - 552. [Abstract] [PDF]

				K Sainio, S. Gilbert, E Lehtonen, M Nishi, N. Kumar, N. Gilula, and L Saxen Differential expression of gap junction mRNAs and proteins in the developing murine kidney and in experimentally induced nephric mesenchymes Development, January 7, 1992; 115(3): 827 - 837. [Abstract] [PDF]

				Y. S. Kanwar, A. Kumar, K. Ota, S. Lin, J. Wada, S. Chugh, and E. I. Wallner Identification of developmentally regulated mesodermal-specific transcript in mouse embryonic metanephros Am J Physiol Renal Physiol, May 1, 2002; 282(5): F953 - F965. [Abstract] [Full Text] [PDF]