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Development, Vol 103, Issue 1 129-134 Copyright © 1988 by Company of Biologists

Journal Articles

Nuclear and cytoplasmic localization of newly synthesized proteins in the early mouse embryo

SK Howlett, SC Barton, ML Norris, and MAH Surani

1-, 2- and 4-cell mouse embryos were labelled with [35S]methionine and the newly synthesized proteins from isolated nuclei were compared with those from cytoplasts and total embryos. There were distinct subsets of translation products present within nuclei compared to those that remained in the cytoplasm. There was no detectable evidence for differences in the presence of newly synthesized proteins in the male and female pronuclei. However, different new proteins associated with nuclei over the time that the embryonic genome becomes active.
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[Abstract] [PDF]


© The Company of Biologists Ltd 1988