spacer gif spacer gif spacer gif spacer gif ARCHIVE ANNOUNCEMENT! spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Link, C. D.
Right arrow Articles by Wood, W. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Link, C. D.
Right arrow Articles by Wood, W. B.

Development, Vol 103, Issue 3 485-495, Copyright © 1988 by Company of Biologists

JOURNAL ARTICLES

Mutant expression of male copulatory bursa surface markers in Caenorhabditis elegans

CD Link, CW Ehrenfels and WB Wood
Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder 80309.

In a search for molecular markers of male tail morphogenesis in C. elegans, we have detected two surface markers that are specifically observed in the copulatory bursa of adult males and the vulva of adult hermaphrodites. These markers are defined by binding of a monoclonal antibody (Ab117) and the lectin wheat germ agglutinin (WGA) to live intact animals. Expression of these markers is dependent on sex, stage and anterior-posterior position in the animal. Four of ten mutants with specific defects in bursal development show altered expression of one or both markers. Because the WGA marker can be expressed in intersexual animals with very little bursal development, posterior surface expression of this marker can serve as an indication of subtle masculinization of hermaphrodites. The timing of expression of these markers is not affected by heterochronic mutations that cause larval animals to express adult cuticles or adult animals to express larval cuticles, indicating that marker expression can be uncoupled from general cuticle development. Mutant lin-22 males, which have an anterior-to-posterior transformation of cell fates in the lateral hypodermis, ectopically express both markers in a manner consistent with a 'posteriorization' of positional information in these animals. These markers should be useful for the isolation and characterization of mutants defective in bursal and vulval development, sex determination and expression of anterior-posterior positional information.


This article has been cited by other articles:


Home page
 J BiochemHome page
S. Natsuka, M. Kawaguchi, Y. Wada, A. Ichikawa, K. Ikura, and S. Hase
Characterization of Wheat Germ Agglutinin Ligand on Soluble Glycoproteins in Caenorhabditis elegans
J. Biochem., August 1, 2005; 138(2): 209 - 213.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Chen, S. Zhou, M. Sarkar, A. M. Spence, and H. Schachter
Expression of Three Caenorhabditis elegans N-Acetylglucosaminyltransferase I Genes during Development
J. Biol. Chem., January 1, 1999; 274(1): 288 - 297.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
J. Maloof and C Kenyon
The Hox gene lin-39 is required during C. elegans vulval induction to select the outcome of Ras signaling
Development, January 1, 1998; 125(2): 181 - 190.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
L M Miller, M E Gallegos, B A Morisseau, and S K Kim
lin-31, a Caenorhabditis elegans HNF-3/fork head transcription factor homolog, specifies three alternative cell fates in vulval development.
Genes & Dev., June 1, 1993; 7(6): 933 - 947.
[Abstract] [PDF]


© The Company of Biologists Ltd 1988